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Table 1 Standard curve results of the determination of CML and CEL in human plasma

From: Quantifying carboxymethyl lysine and carboxyethyl lysine in human plasma: clinical insights into aging research using liquid chromatography-tandem mass spectrometry

Lot number

CML standard curve series concentration (μmol/L)

0.025

0.050

0.100

0.300

0.450

0.700

1.200

1.500

LLOQ

Ranges

curve equations

linearity

1

0.025

0.048

0.104

0.316

0.482

0.702

1.060

1.478

0.025

0.025-1.500

y = 0.140x-0.000076

0.9954

2

0.024

0.056

0.097

0.291

0.475

0.738

1.087

1.461

0.024

0.025-1.500

y = 0.137x-0.001250

0.9928

3

0.026

0.043

0.115

0.270

0.497

0.719

1.114

1.502

0.026

0.025-1.500

y = 0.097x-0.000530

0.9862

4

0.025

0.052

0.089

0.257

0.486

0.725

1.277

1.531

0.025

0.025-1.500

y = 0.081x-0.000298

0.9908

5

0.026

0.045

0.099

0.285

0.501

0.775

1.164

1.382

0.026

0.025-1.500

y = 0.180x-0.000395

0.9910

Mean

0.025

0.049

0.101

0.284

0.488

0.732

1.140

1.471

0.025

   

SD

0.1

0.1

0.1

0.1

0.1

0.1

0.1

0.1

0.1

   

Relative standard deviation (RSD) (%)

3.4

10.8

9.6

7.9

2.2

3.8

7.5

3.9

3.4

   

Relative error (RE) (%)

0.8

−2.4

0.8

−5.4

8.5

4.5

−5.0

−1.9

0.8

   

N

5

5

5

5

5

5

5

5

5

   

Lot number

CEL standard curve series concentration (μmol/L)

0.025

0.050

0.100

0.300

0.450

0.700

1.200

1.500

LLOQ

Ranges

curve equations

linearity

1

0.025

0.048

0.099

0.315

0.506

0.724

1.064

1.414

0.025

0.025-1.500

y = 0.624x-0.000541

0.9928

2

0.024

0.054

0.100

0.281

0.496

0.721

1.116

1.442

0.024

0.025-1.500

y = 0.570x-0.002710

0.9946

3

0.026

0.047

0.090

0.292

0.502

0.727

1.048

1.669

0.026

0.025-1.500

y = 0.354x-0.001160

0.9880

4

0.026

0.048

0.091

0.277

0.486

0.728

1.246

1.496

0.025

0.025-1.500

y = 0.316x-0.001310

0.9936

5

0.025

0.049

0.098

0.289

0.516

0.754

1.118

1.371

0.026

0.025-1.500

y = 0.704x-0.001650

0.9918

Mean

0.025

0.049

0.096

0.291

0.501

0.731

1.118

1.478

0.025

   

SD

0.1

0.1

0.1

0.1

0.1

0.1

0.1

0.2

0.1

   

Relative standard deviation (RSD) (%)

3.4

5.7

5.0

5.1

2.3

1.9

7.0

7.9

3.4

   

Relative error (RE) (%)

0.8

−1.6

−4.4

−3.1

11.4

4.4

−6.8

−1.4

0.8

   

N

5

5

5

5

5

5

5

5

5

   
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BMC Biotechnology

ISSN: 1472-6750

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