Fig. 9

Polyphyllin B promoted ferritinophagy by down-regulating the STAT3/NCOA4 pathway. A PCR was used to detect the expression of STAT3 and NCOA4. B Western blot was performed to detect the expression of STAT3, p-STAT3, and NCOA4. Full-length blots/gels are presented in Supplementary Fig. 2G. C IF was used to detect the expression of p-STAT3. D LDH Assay kit was used to measure the changes in LDH levels. E Western blot was performed to detect the expression of ferritin and LC3. Full-length blots/gels are presented in Supplementary Fig. 2H. *P < 0.05 vs Model; #P < 0.05 vs Model + PB + oe-NC. N = 3 replications/group