Fig. 8

Polyphyllin B promoted CSE and LPS-induced Ferritinophagy in BEAS-2B cells. A The expressions of ferritin and NCOA4 protein were detected by western blot. Full-length blots/gels are presented in Supplementary Fig. 2E. B Calcein-AM method was used to detect the labile iron pool. C The expression of ferritin and LC3 was detected by immunofluorescence double staining. D LDH Assay kit was used to detect the change of LDH level. E CCK8 was applied to analyze cell viability. F The IL-6 and TNF-α levels were detected by ELISA. G The TP53, EGFR, STAT3 and ESR1 proteins were detected by western blot. Full-length blots/gels are presented in Supplementary Fig. 2F. *P < 0.05 vs Control, #P < 0.05 vs CSE, &P < 0.05 vs CSE + LPS. N = 3 replications/group