Fig. 5

mRNA expression of Caspase 3 and protein expression of PARP-1 in untreated vs peptide treated U2OS cells and protein. A Gel electrophoresis expression images of Caspase 3 and B-actin (indigenous control) in treated and untreated cells. Untreated and treated samples of B-actin and Caspase 3 gel bands were run together on the same gel, respectively. Original unedited image of gel can be found in supplementary Fig. 5. B Normalized expression of Caspase 3 in untreated and treated cells shows no statistical change in expression (* P < 0.05, n = 6). C Membrane images of representative cleaved and full-length PARP-1 protein, and GAPDH expression in treated and untreated cells. Original unedited image of membrane can be found in supplementary Fig. 6. D Normalized expression levels of the ratio of PARP-1 full-length protein to cleaved protein. Treated cells showed a decrease in expression of the ratio of full-length PARP-1 protein to the cleaved version (*** P < 0.0001, n = 6)