Fig. 4
Annexin V assay of untreated and IC50 peptide treated U2OS cells. Brightfield and FITC Alexa fluor 488 channel microscopy. A Brightfield and stained untreated cell images (10 × magnifications) displayed a low number of dead cells (circled). B Peptide treated cells showed dominance of green stained cells indicating a progressive significant population of apoptotic population. C Yellow circular highlights were indicative of viable cells, green circular highlights were directive of apoptotic cells, while red circular highlights were prominent in apoptotic/necrotic cells. D Large scale apoptotic cell death was observed in highly confluent treated cells. E Arrow pointer towards budding formed by disrupted cells that is yet to be disrupted and uptake the stain