Fig. 2

Dose dependent cytotoxicity of peptide treated U2OS and L929 cells, affects morphological changes. A Cells were treated with five concentrations of peptide (n = 8). Cells displayed a significant decrease in viability at 100 ug/ml and a greater decrease when exposed both 300 ug/ml and 500 ug/ml treatment (P < 0.05). IC50 value was calculated to be approximately 100.5 ug/ml. B No pattern of cytotoxicity could be observed with increasing concentrations of the peptide screening of L929 mouse cells. C Untreated cells displayed normal morphology of U2OS cells as fusiform in shape with intact membrane in 10 × magnification. D Cells treated with 100 ug/ml of peptide started to become more elongated and sparser, with shrunken volume. E At 300 ug/ml, there was a noticeable deformation appearance of the cells with lose of normal structure. F Treatment with 500 ug/ml of peptide resulted in cells lacking normal morphology, forming rounded cells with disrupted membranes