Fig. 6

Phenotypic analysis of clr-5 mutant in Avicel media with histidine or leucine as sole nitrogen source. Secreted protein and endo-glucanase activity of WT and ∆clr-5 after 7 d of culture on Avicel with histidine (A) or leucine (B) as the sole nitrogen source. Relative mRNA abundance of all CAZyme genes in Δclr-5 mutant vs. WT when grown on cellulose with histidine (C) or leucine (D) as the sole nitrogen source. Cellulase genes are shown in olive, hemicellulase genes are shown in red. E Venn diagram of genes with significantly reduced transcript levels in strain ∆clr-5 relative to WT in response to histidine and leucine. F Enrichment of 296 genes with down-regulated expression in strain ∆clr-5 within the Biological Process (BP) category in GO analysis. Values and error bars represent means and standard deviations of independent triplicate experiments, respectively. Error bars in (A-B) indicate the standard deviations from at least three biological replicates