Fig. 5

Physiological characterizations of N. crassa with leucine or histidine as sole nitrogen source. Consumption of amino acid and cell dry weight of WT and ∆clr-5 when grown in glucose media with histidine (A) or leucine (B) as the sole nitrogen source for 96 h and 48 h, respectively, in round-bottomed 24-well plates. Growth profile of strain ∆clr-5 on agar plate supplemented with histidine (C) or leucine (D) after 60 h of incubation at 25 °C. Error bars in (A-B) indicate the standard deviations from at least three biological replicates