Fig. 3

Transcriptomic profiles of N. crassa Δclr-5 on Avicel. A Differential expression analysis of ∆clr-5 compared with WT strain when induced by 2% Avicel for 4 h. Log2 ratio of ∆clr-5/WT vs. Log10 maximum RPKM in either strain. Up-regulated genes are shown in orange; down-regulated genes are shown in dark cyan. Detailed data are provided in Additional file 2: Table S3. B GO analysis of 371 genes with down-regulated expression in strain ∆clr-5. Total extracellular protein concentration (C), endo-glucanase activity (D), xylanase activity (E), β-glucosidase activity (F), and exocellulase activity (G) of cultures of N. crassa grown in Avicel medium supplemented with 0.75 g/L yeast extract. H Comparison of total extracellular protein concentration and lignocellulolytic enzyme activities of ∆clr-5 relative to that of the WT strain after 4 d of culture in Avicel medium with or without 0.75 g/L yeast extract. Error bars in (C-D) indicate the standard deviations from at least three biological replicates