Fig. 13

The comparative analysis of fluorescent intensities in CTCF for heavy metal mixture treatments and individual heavy treatments. The fluorescent intensities obtained for individual heavy metal treatments of Cd²⁺  + Pb²⁺, Pb²⁺ + Zn²⁺, Cd²⁺  + Zn²⁺, and Cd²⁺  + Zn²⁺  + Pb²⁺are shown in light blue colour bars, light purple colour bars, light pink colour bars and light green colour bars in order. The fluorescent intensities obtained for heavy metal mixture treatments of [Cd²⁺  + Pb²⁺], [Pb²⁺ + Zn²⁺], [Cd²⁺  + Zn²⁺], and [Cd²⁺  + Zn²⁺  + Pb²⁺ are shown in dark blue colour bars, dark purple colour bars, dark pink colour bars and dark green colour bars in order. The error bars represent standard mean differences of three independent biological treatments. A single asterisk (*) represents a P value less than 0.05 (P < 0.05), indicating statistical significance at the 0.05 level. Double asterisks (**) represent a P value less than 0.01 (P < 0.01), indicating statistical significance at the 0.01 level. Triple asterisks (***) represent a P value less than 0.001 (P < 0.001), indicating statistical significance at the 0.001 level. Quadruple asterisks (****) represent a P value less than 0.001 (P < 0.0001), indicating statistical significance at the 0.0001 level