Fig. 1

The genetic architecture of heavy metal sensitive CadA/CadR gene circuit. The CadA gene promoter is represented by a purple colour cylinder. The T7 promoter is indicated by a dark pink colour cylinder. The CadR gene is depicted light green colour cylinder, while the eGFP gene is represented by blue colour cylinder. The Ribosomal Binding Site (RBS) is denoted by a light brown colour cylinder, and the T7 terminators are shown as light blue colour cylinders. The DNA construct spans 1460 base pairs (bp) from the start site (0) to the end site (1460). Various restriction sites are incorporated, including AseI and PacI at the beginning, MluI at the end of the T7 promoter, SphI at the end of the RBS, PciI at the end of CadR, MfeI at the end of the first T7 terminator sequence, FseI at the end of the eGFP promoter, and PaeR7I, SmlI, and XhoI at the start of the eGFP gene coding sequence, as well as BglII at the start of the CadA promoter