Table 3 Plasmids used in this study with two dominant resistance makers under the control of different endogenousC. oleaginosus promoters
Plasmid no. | Plasmid name | Promoter regulating the resistance gene | Resistance gene | Origen |
|---|---|---|---|---|
1 | pRF_GAPDHp-HPH | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) | Hygromycin phosphotransferase (HPH) | Görner et al. [13] |
2 | pRF_D9FADp-HPH | Delta-9 Fatty acid desaturase (D9FAD) | Hygromycin phosphotransferase (HPH) | this study |
3 | pRF_UBIp-HPH | Ubiquitin and ubiquitin-like proteins (UBI) | Hygromycin phosphotransferase (HPH) | this study |
4 | pRF_PPIp-HPH | Cyclophilin type peptidyl-prolyl cis-trans isomerase (PPI) | Hygromycin phosphotransferase (HPH) | this study |
5 | pRF_60Sp-HPH | 60 S ribosomal protein L37 (60 S) | Hygromycin phosphotransferase (HPH) | this study |
6 | pRF_PPIp-APH | Cyclophilin type peptidyl-prolyl cis-trans isomerase (PPI) | Aminoglycoside 3’-phosphotransferase (APH) | this study |
7 | pRF_PPIp-NAT | Cyclophilin type peptidyl-prolyl cis-trans isomerase (PPI) | Nourseothricin N-acetyl transferase (NAT) | this study |