Table 2 Integration of the two dominant markers via ATMT into C. oleaginosus. Transformation efficiency, positive rate, and stability of the mutants are accessed by growth behavior and sequence confirmation. APH, aminoglycoside 3’-phosphotransferase; CFU, colony forming units; Gen, geneticin; NAT, N-acetyl transferase; Nrs, nourseothricin; suc, successive; sim, simultaneous
Dominant marker integrated into C. oleaginosus | Antibiotics added | Picked CFUs from three membranes | Growth on solid media + antibiotics | Growth in liquid media + antibiotics | Sequence confirmed |
|---|---|---|---|---|---|
APH | Gen | 72 | 65/72 (90%) | 32/32 (100%) | 3/3 (100%) |
NAT | Nrs | 72 | 70/72 (97%) | 32/32 (100%) | 3/3 (100%) |
APH/NAT suc | Gen + Nrs | 32 | 32/32 (100%) | 18/18 (100%) | 9/9 (100%) |
NAT/APH suc | Gen + Nrs | 32 | 30/32 (94%) | 16/16 (100%) | 4/4 (100%) |
APH/NAT sim | Gen + Nrs | 17 | 14/17 (82%) | 11/11 (100%) | 4/4 (100%) |