Skip to main content
Fig. 7 | BMC Biotechnology

Fig. 7

From: Expanding the genetic toolbox for Cutaneotrichosporon oleaginosus employing newly identified promoters and a novel antibiotic resistance marker

Fig. 7

Growth curves of double mutants with the addition of both antibiotics (+ Nrs + Gen) in comparison to C. oleaginosus wt with and without the addition of both antibiotics. (a) Five double mutants from successive integration of APH/NAT in comparison to C. oleaginosus wt. (b) Five double mutants from successive integration of NAT/APH in comparison to C. oleaginosus wt. (c) Four double mutants from simultaneous integration of APH/NAT in comparison to C. oleaginosus wt. (d) Average from the biological replicates from (a, b, c), excluding the outliers with delayed growth behavior. APH, aminoglycoside 3’-phosphotransferase; Gen, geneticin; NAT, N-acetyl transferase; Nrs, nourseothricin; suc, successive; sim, simultaneous; wt, wild type

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us