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Fig. 3 | BMC Biotechnology

Fig. 3

From: Expanding the genetic toolbox for Cutaneotrichosporon oleaginosus employing newly identified promoters and a novel antibiotic resistance marker

Fig. 3

Workflow for the genetic modification of C. oleaginosus with an Agrobacterium tumefaciens-mediated transformation. A. tumefaciens cells with a plasmid containing a resistance were used to transfect C. oleaginosus followed by selection on solid media. After restreaking single colonies were used to inoculate liquid media. Finally, the genomic DNA was extracted for PCR amplification of the respective gene and sequencing for validation. Parts of the figure were created with BioRender

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BMC Biotechnology

ISSN: 1472-6750

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