Fig. 4
FGF19ΔKLB exerted anti-fibrotic effect in the liver tissues of Mdr2-/- mice. Four-week-old Mdr2−/− mice were treated by daily i.p. injections of PBS, FGF19WT or FGF19 ΔKLB (n = 5 per group). (A-B) Representative images of Sirius red staining (A) of livers from Mdr2-/- mice treated with PBS, FGF19WT or FGF19 ΔKLB for eight weeks and its semi-quantification (B). (C-D) Masson’s trichrome staining (C) to evaluate collagen levels (black arrows) and collagen volume fractions are determined semi-quantatively by Image J (D). When stained with Sirius Red or Trichrome, the collagen fibers in the periduct look red. (E-F) Hepatic mRNA levels of the pro-fibrotic genes Col1a1 (E) and Col1a2 (F) were evaluated by RT-PCR. (G-H) mRNA (G) and protein (H) levels of TGF-β (G). Mean ± SEM was chosen to represent the data; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; (C, D, E-H) conventional one-way ANOVA, then Tukey. The original blot image is shown in Figure S1