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Fig. 4 | BMC Biotechnology

Fig. 4

From: Development of short hairpin RNA expression vectors targeting the internal ribosomal entry site of the classical swine fever virus genomic RNA

Fig. 4

Efficacy of siRNA and shRNA in HEK293 cells. A Percentage of IRES activity in control and siRNA-treated pCI5 cells (left). Cell viability was measured using the WST assay and indicated by percentage to the OD450 value of control siRNA treated cells (right). Assay was performed after 72 h. Vertical bars indicate the standard deviations. One-way ANOVA and Dunnett’s multiple comparison test were conducted for all samples. P-value < 0.05 is indicated. B IRES activity (left), and WST (right) after 14 days of lentivirus infection to pCI5 cells. Percentages of IRES activity in cells with or without siRNA treatment are indicated. P-value < 0.05 compared with control are indicated (One-way ANOVA and Dunnett’s multiple comparison test)

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BMC Biotechnology

ISSN: 1472-6750

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