Fig. 3

Comparison of shRNA-expressing lentivirus infection with CSFV-IRES activity. A Flow chart of lentivirus infection and assay of IRES and WST. B After 7 days of lentivirus infection, lentivirus vectors were detected under a fluorescent microscope Bz- × 700 (× 200). Translucent and merged images are shown. C IRES activity (left), and WST (right) after 7 days of lentivirus infection. Percentages of IRES activity in cells with or without siRNA treatment are indicated. P-value < 0.05 compared with control are indicated (One-way ANOVA and Dunnett’s multiple comparison test). D After 14 days of lentivirus infection, lentivirus vectors were detected under a fluorescent microscope Bz- × 700 (× 200). Translucent and merged images are shown. E IRES activity (left) and WST (middle) after 7 days of lentivirus infection. The percentages of IRES activity of vector control and siRNA-treated cells are indicated. One-way ANOVA was performed, and a P-value < 0.05 compared with the control is indicated (One-way ANOVA and Dunnett’s multiple comparison test). The amount of luciferase RNA in shRNA-expressing lentivirus-infected cells was measured by qRT-PCR (right). Vertical bars indicate the standard deviations. Representative results of three experiments are shown