Table 1 Primer lists in vector construction
From: Development of a mammalian cell-based ZZ display system for IgG quantification
Primers | Vectors |
|---|---|
P1: 5’-ACTTATGCGGCCGCTGTCGACACCACGCGTAATGCTGTGGGCCA GGACACGCAGGAGGTCAT-3’ | pEF1α-ZZ-PDGFR |
P2: 5’-AACTTCGCGGCCGCCTAACGTGGCTTCTTCTGCCAAAG-3’ | |
P3: 5’-ACATCTGCGGCCGCTGGCTCCGAACAAAAACTCATCTCAGAAG-3’ | pEF1α-ZZ-B7 |
P4: 5’-AACTTCGCGGCCGCTAAAGGAAGACGGTCTGTTCAG-3’ | |
P5: 5’-ATTGCGGCTAGCATGGTGAGCAAGGGCGAG-3’ | pET28α(+)-MC-hTNF |
P6: 5’-ATTCGCGGATCCCTTGTACAGCTCGTCCA-3’ |