Fig. 5
From: Development of a mammalian cell-based ZZ display system for IgG quantification
Identification of monoclonal cells for the high-efficiency production of human anti-TNFα IgG (3f8). (A) The positive cells labeled by the culture media from 45 clones. (B) The dose-dependent IgG binding activity of CHO-ZZ cells to the culture media. CHO-ZZ cells were labeled with a series of diluted media collected from the attached and suspended 3f8-B7 cells. S: media from suspension 3f8-B7 cells. (C) The time curve of IgG secreting by attached 3f8-B7 cells