Fig. 4
From: Development of a mammalian cell-based ZZ display system for IgG quantification
The capturing capability of CHO-ZZ cell to the different species-derived IgG. (A) The histograms of CHO-ZZ cells with IgG capture. (B) The percentage of positive CHO-ZZ cells labeled with fluorescein-conjugated IgG. CHO-ZZ and CHO cells were incubated with an excess of fluorescein-conjugated IgG listed in Table 2 for 40 min at 4℃, washed by PBS, and analyzed by flow cytometry. Hum: human, Rab: rabbit, Goa: goat, Don: donkey, and Mou: Mouse. The data are pooled from three independent experiments, CTR1: CHO cells labeled with FITC-conjugated antibody (a mixture containing human, rabbit, and mouse IgG), CTR2: CHO cells labeled with PE- and cy3-conjugated antibody (a mixture containing donkey, rabbit and mouse IgG), and CTR3: CHO cells labeled with Alexa Fluor 647