Fig. 2
From: Development of a mammalian cell-based ZZ display system for IgG quantification
Displaying ZZ on the surface of CHO cell to capture IgG antibodies. (A) Schematic illustrations of capturing IgG with CHO cells displaying ZZ peptides. (B) Scheme of the ZZ display vectors. SP, signal peptide; ZZ, two tandem Z domains of protein A; TM, transmembrane domain. (C) The scaffold design and display efficiency of pEF1α-ZZ-PDGFR and pEF1α-ZZ-B7. CHO cells were transfected with pEF1α-ZZ-PDGFR and pEF1α-ZZ-B7, respectively. One day later, the cells were collected, labeled with FITC-hIgG, and then analyzed by flow cytometry