Fig. 1
From: Development of a mammalian cell-based ZZ display system for IgG quantification
A plotted standard curve for IgG quantification with CHO-ZZ- cells and its reliability. (A) A non-linear regression plot of the standard curve for IgG quantification. CHO-ZZ cells were incubated with the FITC-conjugated hIgG in the range of 12.5–1000 ng/mL for 40 min at 4℃, and analyzed by flow cytometry. The data were well fitted with three-parameter Hill functions. (B) Linear relationship between IgG binding activity and OD of ELISA. The relative fluorescence intensity of positive cells and the concentration of IgG showed a strong positive correlation. Coefficient of determination (R2) is shown