Table 1 Effect of plant growth regulators and type of callus on indirect de novo shoot regeneration in P. caerulea
Callus type | BAP (mg/L) | KIN (mg/L) | TDZ (mg/L) | PUT (mg/L) | IBA (mg/L) | Regeneration rate (%) | Shoot number | Shoot length (cm) |
|---|---|---|---|---|---|---|---|---|
Leaf | 0 | 0 | 0 | 0 | 0 | 0.00 ± 0.000 | 0.00 ± 0.000 | 0.00 ± 0.000 |
Node | 0 | 0 | 0 | 0 | 0 | 0.00 ± 0.000 | 0.00 ± 0.000 | 0.00 ± 0.000 |
Internode | 0 | 0 | 0 | 0 | 0 | 0.00 ± 0.000 | 0.00 ± 0.000 | 0.00 ± 0.000 |
Leaf | 1 | 0 | 0 | 0 | 0.1 | 90.00 ± 0.000 | 8.33 ± 0.318 | 2.53 ± 0.145 |
Node | 1 | 0 | 0 | 0 | 0.1 | 100.00 ± 0.000 | 8.87 ± 0.233 | 2.67 ± 0.176 |
Internode | 1 | 0 | 0 | 0 | 0.1 | 90.00 ± 0.000 | 8.40 ± 0.200 | 2.23 ± 0.120 |
Leaf | 1.5 | 0 | 0 | 0 | 0.15 | 60.00 ± 0.000 | 6.30 ± 0.208 | 2.30 ± 0.153 |
Node | 1.5 | 0 | 0 | 0 | 0.15 | 73.33 ± 3.333 | 6.90 ± 0.100 | 2.73 ± 0.088 |
Internode | 1.5 | 0 | 0 | 0 | 0.15 | 60.00 ± 0.000 | 6.17 ± 0.145 | 2.20 ± 0.115 |
Leaf | 2 | 0 | 0 | 0 | 0.2 | 90.00 ± 0.000 | 7.67 ± 0.233 | 2.47 ± 0.203 |
Node | 2 | 0 | 0 | 0 | 0.2 | 100.00 ± 0.000 | 7.97 ± 0.233 | 2.73 ± 0.186 |
Internode | 2 | 0 | 0 | 0 | 0.2 | 86.67 ± 3.333 | 7.40 ± 0.173 | 2.53 ± 0.186 |
Leaf | 0 | 0 | 0.5 | 0 | 0.05 | 80.00 ± 0.000 | 8.40 ± 0.252 | 2.40 ± 0.208 |
Node | 0 | 0 | 0.5 | 0 | 0.05 | 93.33 ± 3.333 | 8.67 ± 0.145 | 3.00 ± 0.115 |
Internode | 0 | 0 | 0.5 | 0 | 0.05 | 83.33 ± 3.333 | 8.30 ± 0.100 | 2.20 ± 0.115 |
Leaf | 0 | 0 | 1 | 0 | 0.1 | 60.00 ± 0.000 | 6.33 ± 0.133 | 1.30 ± 0.173 |
Node | 0 | 0 | 1 | 0 | 0.1 | 70.00 ± 0.000 | 6.43 ± 0.267 | 1.80 ± 0.115 |
Internode | 0 | 0 | 1 | 0 | 0.1 | 56.67 ± 3.333 | 6.07 ± 0.133 | 1.17 ± 0.088 |
Leaf | 0 | 1 | 0 | 0 | 0.1 | 43.33 ± 3.333 | 5.40 ± 0.252 | 1.27 ± 0.145 |
Node | 0 | 1 | 0 | 0 | 0.1 | 50.00 ± 0.000 | 5.57 ± 0.167 | 1.50 ± 0.153 |
Internode | 0 | 1 | 0 | 0 | 0.1 | 43.33 ± 3.333 | 5.13 ± 0.120 | 1.13 ± 0.088 |
Leaf | 0 | 2 | 0 | 0 | 0.2 | 50.00 ± 0.000 | 4.73 ± 0.186 | 1.20 ± 0.115 |
Node | 0 | 2 | 0 | 0 | 0.2 | 50.00 ± 0.000 | 5.23 ± 0.088 | 1.40 ± 0.208 |
Internode | 0 | 2 | 0 | 0 | 0.2 | 46.67 ± 3.333 | 4.27 ± 0.186 | 1.13 ± 0.088 |
Leaf | 0 | 0 | 0 | 300 | 0 | 30.00 ± 0.000 | 4.23 ± 0.133 | 1.23 ± 0.186 |
Node | 0 | 0 | 0 | 300 | 0 | 43.33 ± 3.333 | 4.73 ± 0.133 | 1.47 ± 0.120 |
Internode | 0 | 0 | 0 | 300 | 0 | 30.00 ± 0.000 | 4.33 ± 0.186 | 1.27 ± 0.145 |
Leaf | 0 | 0 | 0 | 500 | 0 | 23.33 ± 3.333 | 3.53 ± 0.273 | 1.27 ± 0.176 |
Node | 0 | 0 | 0 | 500 | 0 | 30.00 ± 0.000 | 4.20 ± 0.000 | 1.63 ± 0.088 |
Internode | 0 | 0 | 0 | 500 | 0 | 23.33 ± 3.333 | 3.37 ± 0.120 | 1.30 ± 0.115 |
Leaf | 0.5 | 0 | 0 | 100 | 0 | 20.00 ± 0.000 | 2.70 ± 0.153 | 0.99 ± 0.007 |
Node | 0.5 | 0 | 0 | 100 | 0 | 30.00 ± 0.000 | 3.30 ± 0.200 | 1.20 ± 0.115 |
Internode | 0.5 | 0 | 0 | 100 | 0 | 20.00 ± 0.000 | 2.33 ± 0.233 | 0.83 ± 0.088 |
Leaf | 0 | 0 | 0.5 | 100 | 0 | 10.00 ± 0.000 | 1.87 ± 0.067 | 0.93 ± 0.145 |
Node | 0 | 0 | 0.5 | 100 | 0 | 20.00 ± 0.000 | 2.47 ± 0.067 | 1.10 ± 0.115 |
Internode | 0 | 0 | 0.5 | 100 | 0 | 10.00 ± 0.000 | 1.43 ± 0.133 | 0.87 ± 0.088 |
Leaf | 0.5 | 0.5 | 0.5 | 0 | 0 | 10.00 ± 0.000 | 1.60 ± 0.100 | 0.93 ± 0.145 |
Node | 0.5 | 0.5 | 0.5 | 0 | 0 | 16.67 ± 3.333 | 2.33 ± 0.133 | 1.33 ± 0.176 |
Internode | 0.5 | 0.5 | 0.5 | 0 | 0 | 10.00 ± 0.000 | 1.40 ± 0.100 | 0.83 ± 0.120 |