Table 1 Plasmids and strains used in this study
Strains and plasmids | Description | Source |
|---|---|---|
Plasmids | ||
pET28a | Expression vector, KmR, PT7 | Novagen |
pRSFDuet -pelB | Expression vector, AmpR, PT7 | This study |
pRSFDuet | Expression vector, AmpR, PT7 | This study |
pET28a-DDC | Gene DDC inserted between EcoRI and HindIII sites of pET28a | This study |
pRSFDuet-pelB-aroP | Gene aroP inserted by infusion clone in pRSFDuet -pelB | This study |
pRSFDuet-aroP | Gene aroP inserted by infusion clone in pRSFDuet | This study |
pRSFDuet-pheP | Gene pheP inserted by infusion clone in pRSFDuet | This study |
pRSFDuet-tyrP | Gene tyrP inserted by infusion clone in pRSFDuet | This study |
E. coli strains | ||
BL21(DE3) | Invitrogen | |
BL21-28a-DDC | E. coli BL21(DE3) harboring plasmid pET28a-DDC | This study |
BL21-AB-aroP | E. coli BL21(DE3) harboring plasmid pRSFDuet-pelB-aroP and pET28a-DDC | This study |
BL21-AD-aroP | E. coli BL21(DE3) harboring plasmid pET28a-DDC and pRSFDuet-aroP | This study |
BL21-AD-pheP | E. coli BL21(DE3) harboring plasmid pET28a-DDC and pRSFDuet-pheP | This study |
BL21-AD-tyrP | E. coli BL21(DE3) harboring plasmid pET28a-DDC and pRSFDuet-tyrP | This study |