Table 3 Oligonucleotides used in this study
From: Identification and characterization of sugar-regulated promoters in Chaetomium thermophilum
Name | Oligonucleotide (5’–3’) | Purpose |
|---|---|---|
NdeI-YFP-fw | GCCCATATGCGCAAGGGCGAGGAGCTTTTC | Thermostable YFP |
FseI-YFP-rv | TCTGGCCGGCCTTAAACGCTGGCAGCGTAGTTCTC | |
Ter-4700-XbaI-f | GCCTCTAGATAAGGAAGATGAAGAGAAGCAAGAGG | 5’ terminator |
Ter-4700-MCS-r | GAATTCGGGAAGGCGCGCCAAAACGCGTAGGTATATTAACTTACTCGATACTACTTC | |
Pct_0003950-fw | GCCGAATTCCATAGACTTATAAAACAGTCTGCCCAC | PCBP |
Pct_0003950-rv | GCCCATATGCTTCATCGTAATCTTTTTCGCTTACAC | |
Pct_0011440-fw | GCCGAATTCCTCAGCTTTTCTTGGCAGCTAGATG | PXYL |
NotI-FseI-YFP-rv | GCGGCCGCAAGGGCCGGCCTTAAACGCTGGCAGCGTAGTTCTC | |
Pct_0073860-fw | GCCGAATTCCAACAACCGCAATCTGCCGCAA | PXDH |
Pct_0073860-fw | CAACAACCGCAATCTGCCGCAA | |
no_promoter_fw | AATTCCCTTCATCTGTCTAGAACAACGTGCA | Control linker |
no_promoter_rv | TATGCACGTTGTTCTAGACAGATGAAGGG | |
EcoRI-pAT-fw | GCCGAATTCATGGCAGGCCTTGCGCAACAC | pAT-tag |
Nde-pAT-rv | GCCCATATGATGAGCACCACCTTGAAAATATAAATTTTCAG | |
NdeI-RSA4-f | GCCCATATGGCTACATTAGCCCCTCCACC | RSA4/rsa4 E117D |
NotI-RSA4-Stop-r | GCCGCGGCCGCCTATTAGTTTCTCCATGTCCGAACAGCC | |
5'UR_RpL25-EcoRI-fw | GCCGAATTCTCAGAAACATTAAGTATGTTATGAACCGG | PRPL25-RPL25-YFP |
L25-linker-nostop-NdeI-rv | GCCCATATGTGATCCGACCAGGCCGAGCTTGGTGG |