Table 2 Strains used in this study
From: Identification and characterization of sugar-regulated promoters in Chaetomium thermophilum
Name | Genotype | Purpose |
|---|---|---|
PCBP-YFP | PCBP-YFP, erg1 | YFP expression under control of PCBP |
PXYL-YFP | PXYL-YFP, erg1 | YFP expression under control of PXYL |
PXDH-YFP | PXDH-YFP, erg1 | YFP expression under control of PXDH |
Control | Linker-YFP, erg1 | Testing specificity of the reporter construct |
PXDH-pA-RSA4 | PXDH-pA-RSA4. erg1 | Rsa4 wt expression under control of PXDH |
PXDH-pA-rsa4 E117D | PXDH-pA-rsa4 E117D, erg1 | Rsa4 E117D expression under control of PXDH |
PXDH-pA-RSA4 | PRPL25-RPL25-YFP, hph1 + PXDH-pA-RSA4. erg1 | RpL25-YFP localization in inducible wildtype Rsa4 background |
PXDH-pA-rsa4 E117D | PRPL25-RPL25-YFP, hph1 + PXDH-pA-rsa4 E117D, erg1 | RpL25-YFP localization in inducible mutant Rsa4 E117D background |