Fig. 2

Investigation of sugar-induced transcriptome dynamics in C. thermophilum. a–c The differentially expressed genes (DEGs) after exposure to either glucose or xylose for 6 h are shown. The charts show the significant DEGs as log₂-fold changes according to Illumina deep sequencing of mRNAs (p-adjusted < 0.05). Significant changes in transcripts after glucose induction (3736) compared to the reference condition (a), after xylose induction (4685) compared to the reference condition (b), and comparing xylose with glucose induction (3750) (c). The plotted DEGs are listed in Suppl. Data 1. d Selected genes involved in lignocellulolytic and catabolic function were analyzed with respect to their transcriptional dynamics. Shown are the changes in transcripts as log₂-fold changes after D-glucose (orange) and D-xylose (green) induction in comparison to carbon-deficient reference medium (SPY). The respective gene IDs are given in Suppl. Data 3. e The transcriptome dynamics of selected genes were validated by qRT-PCR. The measured changes in transcripts according to Illumina sequencing (filled bars) are shown alongside changes in transcripts according to qRT-PCR (white bars). Transcript dynamics are compared for a β-xylosidase-like gene (XYL), xylitol-dehydrogenase (XDH), a cellulose-binding protein (CBP) and the gene having the strictest activation in glucose compared to in xylose (CTHT_0011270)