Fig. 1

RNA isolation from C. thermophilum cultures grown in glucose and xylose. a C. thermophilum was grown on a traditional CCM plate for 24 h at 50 °C. From a parental colony grown on a CCM plate, equally sized (approximately 3 mm²) pieces were excised from the periphery and transferred onto fresh plates with either CCM (control medium), glucose-containing medium, or xylose-containing medium. Colony growth was imaged after 20 and 24 h of incubation at 50 °C. Scale bar: 2 cm. b A schematic workflow illustrating the steps of C. thermophilum cultivation prior to RNA extraction. A piece of a parent colony was used to inoculate a liquid SPY culture, which was then incubated for 48 h at 55 °C to deplete a putative cellular sugar reserve. Final cultivation was done in liquid SPY (reference), glucose- or xylose-supplemented SPY at 55 °C for 6 h before RNA was extracted. c The quality of the extracted RNA was evaluated by bioanalyzer measurements. The most intense RNA bands correspond to the intact 25S and 18S rRNA of the reference, glucose- and xylose-induced cultures. The analysis was performed for three biological replicates of each growth condition