Fig. 7

The effects of protein properties on binding affinity. Binding affinities were expressed as Kd (µM), derived from Gibbs Free Energy of molecular docking simulations. A temperature of 298 K was applied. Averages were plotted from triplicate docking analyses, with group sample sizes (n) displayed underneath each bar. Error bars represent SEM. Statistical significance calculations were performed as post hoc Tukey tests following univariate ANOVA. p < 0.05(*); p < 0.01(**); p < 0.001(***)