Fig. 4
From: Expression and characterization of recombinant IL-1Ra in Aspergillus oryzae as a system
Analysis of Asp. IL-1Ra expression and purification is depicted. A SDS-PAGE analysis of the mock transfection control and the recombinant colony. Lane 1: Gangnam stain protein ladder, lane 2: the culture medium of mock transfection control, lane 3: the culture medium of recombinant colony expressing IL-1Ra. B Chromatogram of ion exchange purification using the HiScreenTM Q FF Q column, and SDS-PAGE examination of the fractions selected from the chromatogram. The peaks containing Asp. IL-1Ra and fungal amylase are indicated by arrows in the chromatogram. The SDS-PAGE gel lanes are as follows. Lane 1: Gangnam stain protein ladder, lane 2: the column wash fraction, lane 3: the first fraction of the peak indicated by the black arrow, lane 4: the fraction of the peak indicated by the red arrow. C Chromatogram of size exclusion chromatography using a Superdex75 Increase 10/300 GL column, and SDS-PAGE examination of the fractions selected from the chromatogram. The peaks containing Asp. IL-1Ra and fungal amylase are indicated by arrows in the chromatogram. The SDS-PAGE gel lanes are as follows. Lane 1; Gangnam stain protein ladder, lanes 2–6; the consecutive fractions derived from the first peak (blue arrow) corresponding to fungal amylase; lanes 7–10: the consecutive fractions derived from the second peak (green arrow) corresponding to fungal amylase and Asp. IL-1Ra band around 17 kDa. Full-length gels are presented in Supplementary Figure S1, S2 and S3