Table 1 Process steps during transient expression in N. benthamiana and monitoring measures to detect A. tumefaciens
Process step (–) | Sample type (–) | YEB medium (–) | Unit |
|---|---|---|---|
Infiltration | Air sample | Selective/non-selective | CFU m−2 |
Incubation | Direct contact test | Selective | CFU m−2 |
Blanching | Air sample | Selective/non-selective | CFU m−2 |
Extraction | Liquid | Selective | CFU L−1 |
Bag filtration | Liquid | Selective | CFU L−1 |
Depth filtration | Liquid | Selective | CFU L−1 |
Sterile filtration | Liquid | Selective | CFU L−1 |