Fig. 1

Schematic process flow of A. tumefaciens-mediated transient expression in plants. A Cloning in E. coli, A. tumefaciens pre-culture and fermentation for subsequent infiltration of plants. B Cultivation of wild-type plants, followed by infiltration with the A. tumefaciens suspension and incubation to facilitate transient expression with an optional blanching step before extraction. Downstream processing starts with plant biomass extraction, and includes clarification (e.g., depth filtration), an optional UF/DF step, and purification by chromatography