Fig. 3
From: A critical role for erythropoietin on vagus nerve Schwann cells in intestinal motility
Verification of vagus nerve specific Mpz-EPOR-KO in mice. A PCR genotyping with DNA isolated from the vagus nerve revealed the presence of the 220-bp PCR product, resulting from MpzCre mediated recombination in the Schwann cell of MpzCre-EPORflox/flox mice and not in wild-type (control; 390 bp) and flox/flox (428 bp) mice (n = 3). B qRT-PCR analysis confirms a significant knockout of the EPOR gene in the vagus nerve as compared to control mice. Values were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Unpaired t-tests and data were expressed as means ± SEM, *** P < 0.0002, Control vs. Mpz-EPOR-KO, n = 3