Fig. 3
Evaluation of the company-made dsRNAs for insecticidal activities against F. occidentalis. Two dsRNAs specific to vATPase-A and vATPase-B were produced at a large scale. A Comparative analysis of the insecticidal activities of three different grades of dsRNAs based on purity. A viral gene CpBV302 was used as the control dsRNA. Mortality was assessed at 7 days after treatment (DAT) and corrected by the mortality of control treatment obtained by feeding the control dsRNA. B Dose-mortality curves of the two Grade-1 dsRNAs against adult thrips. Test dsRNA suspensions were applied to the thrips by the feeding method. Each treatment used 10 individuals and was replicated three times. The letters above the standard deviation bars indicate significant differences among the means at Type I error = 0.05 (LSD test) for each developmental stage or target gene. C Median lethal concentrations (LC50) of the two dsRNAs against adult thrips