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Fig. 1 | BMC Biotechnology

Fig. 1

From: Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model protein

Fig. 1

Transient plasmid screen at deep well plate-scale to optimize rRBD titer. (A) Schematic summarizing the elements on the transient expression vectors. The histidine-tagged rRBD gene and associated promoter (rRBD prom) are teal, EBV episomal elements are purple, the optional additional promoter (EBNA1 prom) for EBNA1 is brown, and the ampicillin resistance gene (AmpR) and origin of replication for Escherichia coli (E. coli ori) are grey. (B) Normalized BLI titers for the study comparing culture temperatures (32 °C vs. 37 °C) and promoters (SV40, CAG, CMV) for rRBD expression. (C) Normalized BLI titers for the study comparing culture temperatures (32 °C vs. 37 °C) and EBNA1 gene variants (no EBNA1 [None], repeat-less EBNA1 [X-rep], and wild-type EBNA1 [Full]). (D) Normalized BLI titers for the study comparing EBNA1 expression cassette variants (no EBNA1 [N/A], wild-type EBNA1 [WT] and synthetic SV40 driven wild-type EBNA1 [sSV]). Error bars show SEM for biological and technical duplicates. Corresponding absolute titers used for normalization are shown in Table S2

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BMC Biotechnology

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