Fig. 4

IgG mut-B2 interacted with CTGF through TSP-1 domain. (A) Schematic of full-length CTGF (WT) and its deletion mutants. (B) Binding affinity of IgG mut-B2 to CTGF and its deletion mutants determined by SPR assay. (C) Molecular modeling of variable regions of IgG mut-B2 and TSP-1 domain of CTGF. Left panel: Three-dimensional model of variable regions of IgG mut-B2 structure predicted by homology modeling. The heavy chains’ variable regions are shown in blue and the light chains’ variable regions in green. Right panel: The intermolecular interaction analyses of the IgG mut-B2 with TSP-1 domain. TSP-1 domain of CTGF is represented by magenta. The sticks model is used to demonstrate the key residues involved in the interactions, and the key residues of TSP-1 domain are marked in cyan, key residues of antibody are marked in light orange. Yellow dotted lines represent hydrogen bonds