Fig. 3

Media supplemented with select growth factors, insulin, and C. vulgaris extract supports cell growth in the absence of FBS. Line plots show live (a) and percent dead/non-intact (b) EBTr cells after 0, 2, 4, and 8 days in culture. Different media conditions are indicated by different colors; all counts were performed in duplicate. c Brightfield images show representative EBTr cell density and morphology for each condition and time point; white scale bar indicates a length of ~ 100 μm. CVE was used at 10 g/L and CGF at 10% by volume. Supplements include 10 ng/mL TGF, 30 ng/mL FGF, 3 μM bovine insulin, 0.2 mM L-ascorbate, and 80 nM selenium