Table 1 Purification of A. niger NRC114 α-galactosidase
Purification step | Total units (U) | Total protein (mg) | Specific activity (U/mg protein) | Recovery (%) | Purification fold |
|---|---|---|---|---|---|
Crude enzyme | 3780.57 | 698.32 | 5.41 | 100.00 | 1.00 |
Ammonium sulfate ppt. (60–90%) | 3214.94 | 124.40 | 25.84 | 85.04 | 4.77 |
Sephadex G-100 column | 2981.21 | 12.20 | 244.42 | 78.86 | 45.15 |
DEAE Sephadex A50 column | 1420.65 | 2.13 | 666.02 | 37.58 | 123.02 |