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Table 3 Experimental design and results of the steepest ascent path

From: Production of rhamnolipid biosurfactants in solid-state fermentation: process optimization and characterization studies

Run

Coded variables

Actual variables

Rhamnolipid (g/kg of substrate)

B

D

E

G

B(%)

D(%)

E(°C)

G(mL)

1

0

0

0

0

4

65

30

1

15.78

2

0.25

0.33

0.4

0.3

4.5

70

32

1.15

17.81

3

0.5

0.66

0.8

0.6

5

75

34

1.3

19.33

4

0.75

0.99

1.2

0.9

5.5

80

36

1.45

18.05

5

1

1.32

1.6

1.2

6

85

38

1.6

16.67

6

1.25

1.65

2

1.5

6.5

90

40

1.75

14.32