Table 1 Summary of protease purification
Purification steps | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification (fold) |
|---|---|---|---|---|
Crude protease | 10794.00 | 252.37 | 42.77 | 1.00 |
(NH4)2SO4 precipitation (20–40%) | 7544.35 | 15.30 | 493.09 | 11.52 |
Sephadex G-75 | 3271.58 | 1.65 | 1982.77 | 46.37 |