Fig. 3

N-terminal extension of M.CviQIX has an inhibitory effect on DNA methylation. A Schemes showing recombinant protein structures of M.CviQIX. B Recognition sequence of the restriction enzyme HaeIII. The recognition sequence contains the CC dinucleotide. If the first C is 5-methylated, HaeIII cannot cut the substrate DNA. C Genomic DNA digestion with HaeIII. DNAs extracted from bacterial cells that express the M.CviQIX proteins shown in A were digested with HaeIII and analyzed on E-Gel Ex. D Schemes showing M.CviQIX proteins with or without N-terminal deletions and vector-derived sequences. Tag denotes the vector-derived sequence. E Genomic DNA digestion of bacterial cells using the constructions in D