Fig. 2

Dual-affinity purification strategy used to purify the candidate MTases. A Structure of the expressed protein. B M.CviPI proteins purified only via HisTrap purification and dual-affinity purification combined with HisTrap and StrepTrap purifications. C DNA methylation activities of purified M.CviPI were assayed using the methylation-sensitive restriction enzyme HaeIII. Unmethylated lambda DNA (Promega) was used as the substrate DNA. As a control, M.CviPI purchased from NEB was included