Fig. 2

The CPEs and expression of RFP by the recombinant viruses rZM10 and rZM10-RFP. A DF-1 cells in a 6-well plate were infected with the recombinant viruses at 0.01 m.o.i. the CPEs and the fluorescence of the infected cells were examined and digitally photographed 48 h post-infection, under an inverted fluorescence microscope at 100 × magnification. The DF-1 cells which were not infected was set up as negative control. B Western blotting analysis antibodies response in DF-1 cells infected with the recombinant viruses rZM10 and rZM10-RFP. The molecular weight of HN protein is approximately 87 kDa and was present in the cells infected with the rZM10 or rZM10-RFP. The RFP(~ 30 kDa) protein bands could only be detected in the rZM10-RFP infected cells, confirming the expression of the RFP gene by rZM10-RFP