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Fig. 2 | BMC Biotechnology

Fig. 2

From: Identification of a novel fully human anti-toxic shock syndrome toxin (TSST)-1 single-chain variable fragment antibody averting TSST-1-induced mitogenesis and cytokine secretion

Fig. 2

The expression of five scFvs in E. coli HB2151. The expression of five scFvs, MS457, MS460, MS465, MS473, and MS475, in E. coli HB2151, was evaluated by (A) SDS-PAGE and (B) western blot analysis. (A) The expression level of MS457, MS460, MS465, MS473, and MS475 (~ 27 kDa) in the periplasmic extract of E.coli HB2151 infected with the selected phagemids and the periplasmic extract of uninfected E.coli HB2151 (control) induced by 0.1 mM IPTG were assessed by an SDS-PAGE gel (12%). (B) A single band of the expected size (~ 27 kDa), related to the scFv, was detected by probing with mouse anti-human scFv polyclonal antibody, followed by goat anti-mouse IgG-horseradish peroxidase (HRP)-conjugated antibody. Lane M: pre-stained protein marker. The original images can be found in Additional file 1: Fig. S1

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BMC Biotechnology

ISSN: 1472-6750

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