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Fig. 2 | BMC Biotechnology

Fig. 2

From: Efficient gene delivery into the embryonic chicken brain using neuron-specific promoters and in ovo electroporation

Fig. 2

Neuron-specific promoters in chicken primary brain cells in vitro. A Schematic representation of piggyBac vectors, which express enhanced green fluorescent protein (EGFP, 720 bp) under four different promoters. The CMV promoter is 655 bp. The chicken CaMKII (cCaMKII) promoter is 400 bp. The chicken Nestin (cNestin) promoter is 344 bp. The human Synapsin I (hSynI) promoter is 401 bp. bp, base pairs. B Transfected primary chicken brain cells. Two days after transfection, EGFP expression was visualized by fluorescence microscopy. Scale bar, 50 μm. C DF1 cell line transfected with four vectors expressing EGFP under different promoters. Scale bar, 100 μm

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BMC Biotechnology

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