Fig. 1
From: Generation of a recombinant antibody for sensitive detection of Pseudomonas aeruginosa
A Schematic images of full-sized antibody (middle) and the plasmid DNA maps for the expression of H chain (left) and L chain (right) of recombinant antibody. KZ, SP, VH, CH, VL, and Ck indicates kozak sequence, signal peptide, variable heavy chain, constant heavy chain, variable light chain, and constant kappa light chain. Nucleotide sequence of a kozak sequence and signal peptide for H chain and L chain was 5’-gccaccatgggatacagaatgcagctgctgagctgtatcgccctgtctctggccctggtcaccaattct-3’ and 5’-gccaccatgggatacagaatgcagctgctgagctgtatcgccctgtctctggccctggtcaccaatagc-3’, respectively. B SDS-PAGE analysis of recombinant antibody. “ + ” indicates a sample reduced by adding DTT and heating, and “-” indicates a nonreduced sample