Table 1 Amino acid substitutions accumulated by p450 BM3 DE compared to its parent sequence p450 BM3
From: Oleic acid based experimental evolution of Bacillus megaterium yielding an enhanced P450 BM3 variant
Domains | Mutations |
|---|---|
Heme domain | T1P, V26I, A28T, V127I & A135T |
Linker region | K452Q, P463R, V470E, K473T & A474V |
FMN subdomain | Q546E, L589F, D599E, V624L, D637E & K639A |
FAD subdomain | G660R, T664A, Q674K, T715A, A716T, A741G, A782V, K813E, I824M, E870N & I881V |
NADPH subdomain | E889G, D895G, E947K, E954N, M967V, A1008D & D1019E |