Fig. 2
From: Generation of genome-edited dogs by somatic cell nuclear transfer
Genetic analysis of genome-edited dogs. A Pregnancy was diagnosed using ultrasound on day 45 after embryo transfer (fetuses circled in red). B Comparison of GFP expression in wild-type and DJ-1 knockout (KO) dogs. Image on the left was taken under bright field microscopy, and that on the right shows the expression of GFP. Wild-type dog is in the middle of the photo and DJ-1 KO#1 and DJ-1 KO#2 dogs are on the right and left sides, respectively. C Sequence analysis of the targeted locus in DJ-1 KO dogs. D Analysis of DJ-1 gene expression in DJ-1 KO dogs using real-time qRT-PCR. The X-axis represents the control, DJ-1 KO#1, and DJ-1 KO#2, respectively. The Y-axis represents the mRNA expression level of DJ-1 relative to the control (The data represent the mean values and standard deviations of five reaction replicates). E Western blot analysis of DJ-1 expression in DJ-1 KO dogs. ACTB was used to normalize the expression levels