Table 1 Rabies virus production in CR.pIX suspension cells grown in a 7-L bioreactor using different culture modes
Culturemode | Timeofculture(days) | Workingvolume(ml) | Harvestvolume(ml) | Virustiter(FFU/ml) | GPlevel(µg/ml) | Activity(IU/ml) | Expecteddoses |
|---|---|---|---|---|---|---|---|
Batch | 8 | 2500 | 2500 | 2.3 × 107 | 13 ± 2 | 3.5 | 8750 |
Fedbatch/perfusion | 13 | 4000* | 2000 | 1.9 × 107 | 2.8 ± 1 | 7 | 84,000 |
|  |  |  | 2000 | 1.3 × 108 | 32 ± 12 | ||
|  |  |  | 2000 | 3.2 × 108 | 69.7 ± 5 | ||
|  |  |  | 2000 | 2.5 × 108 | 73.4 ± 4 | ||
|  |  |  | 4000 | 3.3 × 108 | 78.6 ± 10 |